Tbx18 transduction is a method of turning on genes in heart muscle cells as a treatment for certain cardiac arrhythmias. Currently this therapy is in the very early stages of experimentation, having only been applied to rodents. Before this treatment can be used in humans, successful tests on larger animals need to be completed followed by human clinical trials. This treatment is one of the many forms of gene therapy that are currently being researched for use in different diseases.

List of Ozzy Osbourne members

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The Supervet: Noel Fitzpatrick is a Channel 4 television series following the work of vet Noel Fitzpatrick and his team at Fitzpatrick Referrals in Eashing, Surrey. The series shows the work on some of the hardest-to-cure pets from across the country as they receive cutting-edge treatments and surgery from Fitzpatrick and his team, who attempt to cure pets that might otherwise be beyond saving. One series had the Supervet out 'on the road,' mixing up the traditional format, with visits to various dog shows and other types of pets in the community.

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Ricardo López

Erismena is an opera in a prologue and three acts by Francesco Cavalli. It was designated as a dramma per musica. The Italian libretto was by Aurelio Aureli, the only work by this writer for Cavalli. Erismena is the first full-length opera known to have been translated into English and may have been first performed in England in 1674.

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"Soon After Midnight" is a song written and performed by American singer-songwriter Bob Dylan that appears as the second track on his 2012 studio album Tempest. Like much of Dylan's 21st-century output, he produced the song himself using the pseudonym Jack Frost. At less than three-and-a-half minutes, it is the shortest of Tempest's 10 songs and the only example of the pre-Rock Pop Ballad genre to be found on the album. The title is a reference to "fairy time" in William Shakespeare's play A Midsummer Night's Dream.

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The Barents Sea campaign in 1941 was a submarine operation in the Arctic waters of the Barents Sea during World War II. It was a combined Soviet and British campaign, with boats departing from Polyarny to harass German shipping along the Norwegian coast.

Professor Paul Black OBE CPhys FInstP is an educational researcher, physicist and a current Professor Emeritus at King's College London. Black was previously Professor of Science Education and Director of the Centre for Science and Mathematics Education at the Chelsea College of Science and Technology and Head for Educational Studies at King's College London. He is a former Chair for the Task Group on Assessment and Testing and Deputy Chair of the National Curriculum Council, and is recognised as an architect of the national curriculum testing regime and the national curriculum for Science.

Ozzy Osbourne is an English heavy metal vocalist from Aston, Birmingham. After he was fired from Black Sabbath earlier in the year, Osbourne formed a solo band (initially known as Blizzard of Ozz) in November 1979 with guitarist Randy Rhoads, bassist Bob Daisley and drummer Lee Kerslake. Since their inception, the group's personnel have changed regularly. The current lineup includes guitarist Zakk Wylde (who first joined in 1987 until 1992, spent a second tenure in the band from 2001 and 2009, and most recently rejoined in 2017), bassist Rob "Blasko" Nicholson (a member since 2003), keyboardist and rhythm guitarist Adam Wakeman (who first joined as a touring musician in 2004, and was made an official member with the release of Scream in 2010), and drummer Tommy Clufetos (since 2010).

Spanish Americans (Spanish: estadounidenses españoles, hispanoestadounidenses, or hispanonorteamericanos) are Americans whose ancestry originates wholly or partly from Spain. They are the longest-established European-American group with a continuous presence in Florida since 1565.

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February 9 is the 40th day of the year in the Gregorian calendar. 325 days remain until the end of the year (326 in leap years).

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The 2017–18 Arizona State Sun Devils men's ice hockey season was the 3rd season of play for the program at the Division I level. The Sun Devils represented Arizona State University and were coached by Greg Powers, in his 8th season.

Soon After Midnight

David Werner Amram III

Geordie Shore is a British reality television series broadcast on MTV and based in Newcastle upon Tyne, England. It was first broadcast on 24 May 2011, and is the British offshoot of the American show Jersey Shore. The programme follows the daily lives of 8–12 housemates, as they live together for a number of weeks. In the first series, the house was located in Jesmond, a suburb of Newcastle. Since the second series, the show has used a house in the Oceana Business Park in Wallsend.

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The following is a list of notable deaths in November 2020. Entries for each day are listed alphabetically by surname. A typical entry lists information in the following sequence:
* Name, age, country of citizenship at birth, subsequent country of citizenship (if applicable), reason for notability, cause of death (if known), and reference.

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Michael Davis (born July 1946) is the founder of businesses and non-profit foundations supporting the resolution of political conflicts. His book Life after Democracy suggests a new relationship between political leaders and the public to reverse the global rise of violence and authoritarian controls.

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Eparchies and Metropolitanates of the Russian Orthodox Church

The Supervet: Noel Fitzpatrick is a Channel 4 television series following the work of vet Noel Fitzpatrick and his team at Fitzpatrick Referrals in Eashing, Surrey. The series shows the work on some of the hardest-to-cure pets from across the country as they receive cutting-edge treatments and surgery from Fitzpatrick and his team, who attempt to cure pets that might otherwise be beyond saving. Filmed using fixed rig cameras in the clinic's waiting room, reception and prep area, the programme captures the raw emotion, drama and playfulness of Fitzpatrick and his staff as they treat the pets in their care. One series had the Supervet out 'on the road,' mixing up the traditional format, with visits to various dog shows and other types of pets in the community.

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Protests in Russia began on 23 January 2021 in support of the opposition leader Alexei Navalny after his arrest and the release of the film Putin's Palace, which revolves around the connection between President Vladimir Putin and a palace allegedly being built for him. On the first day, protests were held in 198 towns and cities across Russia in what were one of the largest anti-government demonstrations since the 2011–2013 Russian protests.

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Protests in Russia began on 23 January 2021 in support of the opposition leader Alexei Navalny after his arrest and the release of the film Putin's Palace, which revolves around the connection between President Vladimir Putin and a palace allegedly being built for him. On the first day, protests were held in 198 towns and cities across Russia in what were one of the largest anti-government demonstrations since the 2011–2013 Russian protests. On 2 February, Navalny's suspended sentence of three and a half years was replaced with a prison sentence, meaning he would spend over two and a half years at a penal colony. On 4 February, Leonid Volkov, Navalny's chief of staff, stated that they did not plan to hold further protests until the spring or summer to focus on the upcoming parliamentary elections later in the year. He also said that Navalny's team would use "foreign policy methods" to pressure the government into releasing Navalny. On 9 February, Volkov announced new courtyard protests on 14 February, shifting from street protests to avoid direct confrontation with police.

Paul Black (educational researcher)

2017–18 Arizona State Sun Devils men's ice hockey season

Charles Boyer (French: [bwaje]; 28 August 1899 – 26 August 1978) was a French-American actor who appeared in more than 80 films between 1920 and 1976. After receiving an education in drama, Boyer started on the stage, but he found his success in American films during the 1930s. His memorable performances were among the era's most highly praised, in romantic dramas such as The Garden of Allah (1936), Algiers (1938), and Love Affair (1939), as well as the mystery-thriller Gaslight (1944). He received four Oscar nominations for Best Actor.

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2021 Russian protests

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This is a list of all Open Era tennis Grand Slam singles champions and how old they were when winning their first title.Players who won a title before the Open Era are designated with an asterisk (*), but those results do not factor into these lists.

The third season of The Amazing Race (also known as The Amazing Race on Space) is a Latin American reality television game show based on the American series of the same name, The Amazing Race. This season was the first to air on the Space channel in association with Disney Media Networks Latin America. It featured eleven teams of two, with a pre-existing relationship, in a race across Latin America to win US$250,000. Harris Whitbeck returned as the host for this third season. The third season premiered on 25 September 2011 at 9:00 p.m (UTC-3) on Space and TNT. The season finale aired on Space and TNT on 18 December 2011 at 9:00 p.m (UTC-3). Chilean twins Cristóbal and Nicolás Brain were the winners of this season.

Barents Sea submarine campaign (1941)

David Werner Amram III (born November 17, 1930) is an American composer, arranger, and conductor of orchestral, chamber, and choral works, many with jazz flavorings. He plays piano, French horn, Spanish guitar, and pennywhistle, and sings. Amram has also composed scores for films, and has led quartets, quintets and larger ensembles who perform and record under his name.

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The third season of The Amazing Race (also known as The Amazing Race on Space) is a Latin American reality television game show based on the American series of the same name, The Amazing Race. This season was the first to air on the Space channel in association with Disney Media Networks Latin America. It featured eleven teams of two, with a pre-existing relationship, in a race across Latin America to win US$250,000. Chilean twins Cristóbal and Nicolás Brain were the winners of this season.

This is the list of the metropolitanates and eparchies (dioceses) of the Russian Orthodox Church.

The Supervet: Noel Fitzpatrick

Tbx18 transduction is a method of turning on genes in heart muscle cells as a treatment for certain cardiac arrhythmias. Currently this therapy is in the very early stages of experimentation, having only been applied to rodents. Before this treatment can be used in humans, successful tests on larger animals need to be completed followed by human clinical trials. This treatment is one of the many forms of gene therapy that are currently being researched for use in different diseases. Tbx18 gene therapy is aimed at treating a group of arrhythmias known as sick sinus syndrome. In a healthy heart, sinoatrial (SA) nodal cells act as the heart’s pacemaker and cause the heart to beat in a regular rhythm. Approximately 10 thousand of the 10 billion cells in the heart are SA nodal cells. Although they make up a relatively small portion of the heart SA node cells play a crucial role in the heart’s function. The problem in sick sinus syndrome is that the SA node is not functioning properly and is causing an irregular heartbeat. Currently the treatment for sick sinus syndrome is to remove the SA nodal cells that are not functioning properly (?) and to implant an electronic pacemaker to maintain a regular rhythm. The Tbx18 gene is required for development of pacemaker cells in the heart during fetal development but is normally not functional after birth. Expression of Tbx18 after birth requires adenovirus vectors to deliver the gene into the atrial myocytes. Tbx18 transduction converts atrial muscle cells into SA node cells that initiate the heartbeat. An engineered virus carrying the Tbx18 gene is injected into animals and infects atrial muscle cells. Inside atrial muscle cells the Tbx18 gene is expressed. Tbx18 turns on genes that drive SA node cell development, simultaneously turning off genes that create atrial muscle cells. Tbx18 gene therapy has been successful in rodent hearts, converting atrial muscle cells into SA node cells by expression of the Tbx18 transcription factor. Tbx18 expression in atrial myocytes was shown to convert them into functional SA nodal cells in an experiment done in rodents. These converted SA node cells are able to respond to the nervous system, allowing the heart to be regulated as normal. Adenoviral TBX18 gene transfer could create biological pacemaker activity in vivo in a large-animal model of complete heart block. Biological pacemaker activity, originating from the intramyocardial injection site, was evident in TBX18-transduced animals starting at day 2 and persisted for the duration of the study (14 days) with minimal backup electronic pacemaker use. Relative to controls transduced with a reporter gene, TBX18-transduced animals exhibited enhanced autonomic responses and physiologically superior chronotropic support of physical activity. Induced sinoatrial node cells could be identified by their distinctive morphology at the site of injection in TBX18-transduced animals, but not in controls. No local or systemic safety concerns arose. Thus, minimally invasive TBX18 gene transfer creates physiologically relevant pacemaker activity in complete heart block, providing evidence for therapeutic somatic reprogramming in a clinically relevant disease model. The currently used electronic pacemakers have drawbacks such as equipment malfunction, limited battery life, lack of nervous system regulation, and risks associated with implantation of the device in one’s chest. Creation of a biological pacemaker could prove to be a feasible alternative that eliminates some of the problems associated with electronic pacemakers. Various gene and cell-based approaches of creating a biological pacemaker have been looked at over the last few years. The method of turning on Tbx18 genes in heart muscle cells is a new method being researched that, so far, has shown promise for being effective.

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Tbx18 transduction